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Welcome to Flow Stars, candid conversations between Dr.

Peter O'Toole and the big hitters of Flow Cytometry,

brought to you by Beckman Coulter at Bite-size bio.

Hi, welcome to Flow Stars,

a very special edition recorded live with a live audience at Cyto

2023. We feature Jessica Houston, Paul Wallace,

and Pip Chatter Power Day,

and we talk about where they see the utilization of flow cytometry going

over the next 10 years.

It's a hard question, but, um,

I think that it's probably going to stay the same because

we're gonna have more technologies to choose from.

And so there's gonna be a little bit of a drag on,

on the number of people doing flow. But, um, the,

the flip side of it is that actually doing a flow cytometry experiment is

infinitely easier than it used to be. And data analysis is something that,

you know, we're all focusing on. And so, you know,

I think that's gonna make it easier to take up flow.

So those two trends are probably gonna cancel each other out,

Including different opinions.

I don't think there's any question that the utilization will increase. We,

we do see changes. We see, you know, uh,

maybe CD fours are no longer needed as much as they were. Uh,

there's definitely a transition with MRD going more to next gen sequencing,

but there's always, I mean, there are always new, uh, techniques coming out,

new applications coming out, and, you know,

we're moving into a world of microvesicles, and I just,

I just see that as continuing on for,

For a long time.

And is conventional flow cytometry dead?

No, it's not dead. I think there's, so, I mean, we had a good example of the,

at the meeting with, um, resource poor places where the,

it's a critical facility, so you know, the complexity, you know, spectral,

whether you wanna say it's complex or not. I mean, there's,

there's still a lot of places in the world that need just, you know,

the standard simple method,

Oh, in this very special live episode of Flow Stars.

Hey, ladies and gentlemen, we're about to start.

First off,

I'm gonna say a quick thank you to Beckman Kolter for not entertaining you this

evening and letting flow cytometrists entertain you instead,

which is a bit different, isn't it? Okay, so it is live flow stars,

and we've got three very special guests tonight. We have Paul Wallace,

Jessica Huston.

Alright, Jessica

And pr, Jessica re even Jesus Christ name, mate.

Okay, so I'm Peter at all from the University of York.

I am not working for Beckman kta. Okay. So don't get that confused, please.

Okay, so let's kick off. I'm gonna ask our guests different questions,

different questions you have also messaged in questions. Okay?

So some of those questions I will call your name out, and I will say, actually,

if you are embarrassed by your question, tough luck. Okay?

So my first question, it's,

which company does the best cyto party? Uh,

Oh,

I'm gonna stop

Peter. I have to say I love 'em all. I mean, they're, I mean, you, you can,

I mean, there, there are, there are definitely a lot of good parties, but the,

the, there are a lot of good parties at, at sto,

but the company parties are the best.

That big culture.

Jess. Jessica, I'd just like to remind you this is being recorded.

Yes. Okay. I know. Would

You, would you like to say any details? I be

More diplomatic about that.

Jess Jessica is the next president,

so really she should say that ISAC throws the best parties. Yes.

I don't know about that. I

Don't have any conflict of interest. And you've put me up here.

I can say that culture throws the best part. There you go.

So,

Okay. So the next question actually comes from Johnny Moore Uhoh.

So Johnny asks, what is the best cyto that you've actually attended?

Oh, the best.

Oh, I'm gonna start that in this time. pr

So I have, um, you know, I have a selfish answer, but Vancouver was my favorite.

Alright. Alright.

You know, we,

we managed to pull together a meeting that was heavily focused on single

cell biology, and, you know, that's who we are.

And it was so great to see it in different ways. And Vancouver is really pretty.

Um, so this is probably my second favorite.

Wow, that's a good one. And I, I have to pull the selfish card as well.

Montreal. This one is my favorite so far. All right. I feel like, yeah, as,

What was that, Jessica?

This one, this one, this one, this one. This one? Yeah, this one. I, I mean,

obviously it's not over yet. We have one more day to go, but I think,

and we have

A party to go.

And we have a party. Yes, we do. But I, yeah, we go. No, I, I, I,

I think this one's gone well, and I'm really happy about it. And I'm glad that,

um, all the content we have and the plenaries and have, have,

are been well received. So, yeah, I have to go. So,

Jessica, who's in charge of this one? This,

That would be me. Yeah.

There's no conflict here, is it

At all?

So, Paul, now you know that Jessica's in charge of this one.

What I know is that Johnny asked the question, so is Philadelphia, which is my,

which is why, which is why Hometown, but I think outside of Philadelphia,

which would have to be number one, it would be, uh, Quebec City. Uh,

that wa that was, that was a, that was a fabulous one.

And Montpelier, I mean, ILI like the Montpelier ones, but Seattle,

which is the one that I hosted, uh, was would be one.

Okay. So actually, another question that came in is,

why do you keep coming back to Cyto?

I'm going Jessica, first,

I, it's, I mean, ISAC is my home. It's my scientific community.

And I, you know, the cyto meeting is so pinnacle in term for,

for the society. And, you know, I, I like,

I obviously I have an obligation now. It's why I'm coming back, but, um, it's,

yeah.

You feel obligated, huh?

Yeah, I, you know, it's, I I am, uh,

all of the work I've done with,

through the society and the meetings and the contributions we made in our lab

to the, you know, the, the, the meeting is. Yeah, I, it's a hard question. I,

I just come back because this is, this is a home, it's a community. It's family.

Yeah.

Okay. I'm Paul.

Well, I mean, I, I, I personally love the plenaries and the, the,

all the, uh, the sessions.

But I think the reason I come back is 'cause of the people I come back to see

you guys. I mean, it really,

it is a lot of fun just reconnecting with a lot of old friends,

Everyone who is just someone here you'd rather not see

Everybody here

Petite. Now you've got, you are now here. You, you are now going commercial.

Yeah. So does that make it different? Yeah. Coming to society.

Yeah. So it totally makes it different. You know, my,

my experience has evolved because first it was really to scratch the itch,

to teach flow cytometry. And I got to do that in a,

in a forum where people really appreciate education and teaching. And so,

you know, that was really exciting and, you know, kind of empowering to me.

And then it was, you know, to present my cytometry work. And so, you know,

and now it's become more to come and connect with, um,

with vendors and people doing exciting new science who are in the commercial

space. And, you know, I,

I'm almost embarrassed to say that I'm spending more of my time, you know,

planning out new collaborations and, and new partnerships with commercial, um,

entities rather than going to sessions and, and plenaries.

And so I gotta figure out a way to clone myself,

but maybe we'll have a new company around to do that. So

Pr Thank you. So a question from Chris Hall. Now, this is, this is,

this is gotta be the best question that I actually read this morning.

So I recently discovered, said, Chris,

that accepter the acceptor in tandem dye Pacific Blue is a sunscreen.

Have you ever tried adding sunscreen to cells to analyze on your instruments?

And that's a great question, wasn't it?

And what other household items have you run through your flight cytometers?

I have a, I have a story about this. So my, my neighbor, um,

had a, there was a leak coming from his yard that was totally flooding our yard,

and I was convinced it was from his pool water.

And we had just gotten our CyTOF a few years ago.

And so I quietly took a sample of the water and I ran it on CyTOF,

you know, with a control like, well,

rainwater control and a tap water control and compared it.

And I couldn't tell what was going on.

So pool water that I thought, you know,

that allegedly my neighbor dumped at tar yard

Pool, Jessica, household items you put through a place to,

I I just, I

Don't think about that. Well, we put, we all put bleach through it. Um,

but I guess, uh, the, the oddball once was milk. I was trying to, uh,

use that, uh, as an absorbent to get rid of some autofluorescence and, uh,

uh, Coca-Cola. Oh, Coca-Cola.

I was just curious as to what it would do. Ah, didn't do much.

How many times did you have to prime or purchase

Right after? No, it's actually, it's a cleanse. I mean, you know, it, it,

all those bubbles, you know, the interesting,

Ah, that's bizarre. Okay, next question is from Claire Brown. Okay.

What was the most challenging career transition for you?

So I might start actually this time.

Um, it was, uh, they were, they were all challenging,

leaving Mario's lab was, you know, like growing up and leaving home. So it was,

um, that was definitely hard. But, um, I th I think the hardest transition was,

you know, running my own business because then, you know,

it was a small business. I was cleaning toilets, I was, you know,

doing everything. And, um, you know, there's,

it's nice not having a biosafety committee or, you know,

purchasing group or something like that. You can do whatever the heck you want,

but it's, um, it's also taxing. And, you know,

it gets exhausting really quickly. So

Cleaning toilets, it's like, oh, I've even gotta clean the toilets. Now.

If you ever came to University of York,

I wish I was allowed to clean the toilets. Yeah. Really?

Yeah.

'cause I do a lot better job than the cleaner does. Trust me. It just,

it just agrees me, I can't do it. I could do it if I was allowed to.

It's not like I can't clean the toilet.

You can come to my lab and clean the toilets. Gosh,

Have we got a job for you?

I'll get the ONGs. Uh, Jessica, difficult transition,

Todd. Yeah, for me it was definitely postdoc to faculty position. That's,

you know,

you become a faculty and it's a bit of a deer in the headlights experience.

But I,

the reason why I was most challenging is 'cause when you're postdoc and you

decide to start a research program, you wonder, what am I gonna do?

And really it was, should I take what I'm doing as a postdoc and continue?

And I came from Los SMOs National Lab,

we were developing that technology and flow and brought it to NMSU,

and I talked to the guys there, and should I do this? It's like, yeah, why not?

You know? And for me it was like, you know, I didn't like translate that. Yeah,

this is my work. I can continue it and maybe bring in new aspects to it.

But that was the hardest part until I got a comforting, like, yeah,

do it. Mm-Hmm.

Yeah. I think, I think most people can empathize Yeah. With that. And Paul,

So as you all know, I'm a little bit senile, so I,

I only can think about what is the most current, would

You like me to repeat

The question? Yes, that might be a good idea. But I, the, the, uh,

as I, as I went into retirement,

so announcing that I was gonna retirement and then no longer being relevant to

the, uh, to the organization or to the staff, was probably, was,

was very difficult. But actually retiring and then having,

not a whole lot to do. And fortunately,

I flunked retirement and then joined Cyros. So I think the, uh,

the flunking of retirement was probably the, uh, the hardest transition.

Okay. So that brings me really nicely onto,

one of the questions I had for you as well was, when you do properly retire,

will you ever look at a flows cytometer again? I'll start April.

I, I would, I've always wanted to have one of my basement, uh,

To cleaning out your Coca-Cola. Yeah,

Well, there's just, just to run things through. I mean, I, you know, I,

I couldn't imagine pollen, uh, microparticles.

I would love to have one in my basement. So, Beckman Coulter, um, if you have,

if you've got any, if you've got any, uh,

things that you want to use as anchors, um, I got a place to anchor it.

Jessica?

No, no instruments for Paul.

Yeah. One of these days when I retire, I, I,

I would like to have some lasers hanging around. Yeah.

And maybe play around with that. I, maybe not like, you know, a flow cytometer,

but things I couldn't tinker with. That'd be

Fun. Yeah. So just lasers. Are you gonna put some cart lasers, detectors,

some smoke in the room, then just someone sits around's, just

Shoot at the planes? Yeah, the planes will fly overhead.

I don't know, something like that. I don't know.

So, um, you know, I remember when I was in graduate school, there was a, um,

a professor named Arthur Denberg who was in his nineties,

toddling around the hall doing TV work.

And so I told my wife that I'm gonna art denberg it, you know,

I'm just gonna go right to the end. You know, my bony body's gonna have, uh,

you know, a, a lab coat barely hanging off of it, and I'm gonna have a blast,

you know, uh, people might think I'm crazy, but you know who caress, right.

So,

Okay. So next question comes from Peter Lopez. Now this is a bit more serious.

Could you predict how the utilization of flow cytometry iss going to go in the

next five to 10 years gonna go up, gonna go down or stay the same? Gosh,

it's like, play your cards right? Higher, lower stick, uh,

petite,

Uh, it's, you know, it's a, it's a hard question, but, um,

I think that it's probably going to stay the same because we're gonna have

more technologies to choose from.

And so there's gonna be a little bit of a drag on,

on the number of people doing flow. But, um, the,

the flip side of it is that actually doing a flow cytometry experiment is

infinitely easier than it used to be. And data analysis is something that,

you know, we're all focusing on. And so, you know,

I think that's gonna make it easier to take up flow.

So those two trends are probably gonna cancel each other out.

Okay. And go ahead, Jessica, go. Sure.

Yeah. I mean, I would probably challenge Peter to say like,

what do you mean by up down? I'll give 'em the engineering answer, which is,

you know, that, are you talking about how many, how much more use,

or in what way is it going to go up? Because Yeah, that's, you know,

that's hard to answer.

Okay. Yeah,

Paul, I don't think there's any question that the utilization will increase. We,

we do see changes. We see, you know, uh,

maybe CD fours are no longer needed as much as they were. Uh,

there's definitely a transition with MRD going more to next gen sequencing,

but there's always, I mean, there are always new, uh, techniques coming out,

new applications coming out, and, you know,

we're moving into a world of microvesicles. And I just,

I just see that as continuing on for, for a

Long time.

So, okay. So I, my my follow up question to that is,

conventional flow cytometry dead,

and is spectral the only way forward five years time?

Is anyone gonna buy a non spectral system? That's my a quick question. Yes. No,

Jessica.

No, it's not dead. I mean, there's, so, I mean, we had a good example of the,

at the meeting with, um, resource poor places where the,

it's so critical to still use it. So, you know, the complexity, you know,

spectral, whether you wanna say it's complex or not. I mean, there's,

there's still a lot of places in the world that need just, you know,

the standard simple method. I don't, I don't think it is. I mean,

maybe, I don't know, 20 years out, 30 years out,

eventually it might get there, but not yet.

Okay.

Um, was it, mark Twain said, rumors of my death have been greatly exaggerated.

I, I think that's applicable to flow cytometry because, um, you know, we,

we thought that with the emergence of mass cytometry,

there was no reason to do flow anymore. Um, people are doing flow still. Um,

we thought with the emergence of sighte and, you know,

high parameter protein analysis through site seek,

we wouldn't have to do flow anymore. And we still do flow. And so, you know,

it's, uh, I think it's here to stay.

Okay. Um, cool.

I mean, I'm a big fan of spectral, so I, I, I, my first thought was, oh,

spectral is gonna take it over. But then as you think about it,

there's absolutely a lot of applications that are just one color.

We don't really, we're really not, we're really maybe not all that aware of it.

I mean, we, we sort GFP and that type of thing. But out there,

you think about the beer industry and there,

there's a lot of people that are looking at yeast and, and, and using, using,

uh, sort, uh, using instruments for that within the, uh, um,

there's a whole lot of sorting going on, on sperm, uh,

you know, for, for sexing. I mean, that's, there's,

there you hear about places that have a thousand instruments, uh, just running,

uh, running these things. And then, uh, uh, you know,

within the milk industry, looking at mastitis, it's, it's so, you know, I, I,

while I honestly think that where a lot everybody in this room is going as

spectral, there is a lot of industry out there that will be single color.

Okay.

So what is your favorite tool or instrument in the lab?

And I'll start this, I'll start with Paul.

Oh my gosh. Um,

Not a cytometer.

What is your favorite tool or instrument other than a cytometer in the lab?

That's a, that's a tough question. Um,

I, I know, I know that, uh,

I had a couple working in the lab, uh, and at night,

I think their favorite instrument was the centrifuge. Um, and,

and that was for the piper. Anyway, the, uh, I, I,

I would imagine the, uh,

but the one that comes to mind is the eight channel pipette.

But I have really no, no, uh, real answer.

I think it was the idea of just doing the single and being able to move things

into eight channel. So that's a, that's a,

If you had an eight channel pet, you think a politician would look really cool.

You think, my God, they can actually hold a ppl if there's an eight channel one,

they must be really good politicians. Yeah. Uh, petite,

You know,

those little fugees that are like this big that you spin the micro fuge tubes in

and you just close the thing and you don't have to press anything as su you

close it, it spins like a maniac, and you've got the plastic cover on it,

and so you can watch it spin and slow down that. I mean,

if I weren't a scientist, I would just like, enjoy the heck out of that. So,

children's toy, maybe

Jessie, I,

I have to say the oscilloscope, I mean,

I just get so giddy when I'm like tweaking that. That's good.

Teaching students how to do it and look at this waveform and, you know,

you measure the velocity and, and you know, full width half Maxs, and they,

they, you know, they're like, this is weird.

I'm a chemical engineer and I shouldn't be learning this. But yeah, so I, I I,

the oscilloscopes and the function generators,

we can play around with different types of ways to modulate the laser and it's

fun.

So thinking about oscilloscope, I've gotta ask,

what was your favorite flow cytometer of all time?

Jesse? Wait, say that again? What was your favorite flow? Cytometer of all time.

All time. The fax vantage Hands up. Yeah, hands down. I mean, I just,

the fax vantage for me has been so fun to work with.

I can take out the, I can open it up, you know,

unplug from the PMT put in new kept

Pre

Pre Amplifications. Huh?

Is that 'cause it kept breaking, sorry. We, yeah,

It's a fun tool to teach students on and, you know, to, to modify.

We do modifications of cytometers. Okay.

Fat fatigue. Your favorite close cytometer of all

Time. Um,

no comments because there is no reason for me to anger any of my

beautiful ex-girlfriends. You know, it's like, right. It's, um, it's, you say,

you say good things about your symphony and then you're like, yeah,

but the Bigfoot is awesome. But yeah,

I really had good times on the Coulter in 1996 or something, but, you know,

that I learned post cytometry on, so, you know,

there's no answer to that question. Nice driver.

I fell through that.

Alright. I'd have to go with the epic five. That was one,

that was one of my first instruments. And I love being able to play around. And,

you know, you, you, you could pull out filters easily,

you could do a lot of manipulations with That was, that was,

that would be my favorite

Mo Molo legacy. Mm-Hmm. Come on. Did you not have a Molo legacy?

Was it that,

was it the really big one that had like a table that went all the way around?

Ah, and the telephone switchboard on the side. Ah.

So when the engineer came,

you could just turn things around and completely confused the hell outta the,

for ages. John, what was your favorite, what's the legacy?

Legacy? Who had a, who had a leg? Hands up. You've had a mo flow legacy.

No. Keep your hands up.

Put your hands down if it wasn't your favorite instrument. There you go. See,

look at all the hands still up. Okay, so Batman quarter,

can you please bring back the legacy,

but can you just let it do six way sorting as well? Okay.

So the next question from Claire Brown, what do you most love about your job?

And I'm gonna start again with Jessica on this one. Uh,

It's to say the flexibility, uh, just being a faculty,

you can create your own hours, design your own research.

You interact with students every semester. They're always, you know,

it keeps you young. Yeah, I, I flexibility.

Okay. And I'm gonna go to Paul next.

So for me, it was working with the students, no question at all.

I enjoyed really working with the students, talking about the research going on,

you know, about, uh, different experimental design, reviewing the data.

You know,

there was all the hassles and politics and everything else that was going on,

but, you know, you could sit down and, and talk science with, uh, with, with,

with the students. And that I love the most.

Okay.

No question about that.

Fati,

I think, um, being in the lab alone, late at night,

finishing up a set of samples that, you know,

on the cytometer and you just blast horrible music that nobody else would

tolerate. And, you know, and you're in a zone then and you're just, you know,

the experiment's done and you're just working through and you're playing with

the, you know, playing with the, um, the results on the screen. It's great.

It's, it's like, you know, that that's my heaven. Well, sort of.

Okay, so I, this is a difficult question set by, uh, Evan Ellison.

What accomplishment, infl cytometry are you most proud of?

I'll start fatigue this time.

Uh, I mean, this is an easy question for me. It's, it's the, uh,

the introduction of the high parameter, you know,

really high parameter flow doing the, um,

going into Mario's office with the first results from a 1718 color flow panel

and just showing it to him. And I did it just kind of on a lark and, you know,

his eyes lit up and he was like, you just did the first 17 color experiment and,

you know, you,

you can't get those moments in science just as a young scientist where,

you know, your, your hero is telling you you did something cool. And, um, and,

and that was the, you know, the start of what I built my career on. So,

And who,

Uh, well, I, I was very early in my career,

I was working at a company called SynXis. And, uh, they were ma they made the,

uh, lipophilic tracking dyes, the PKH dyes. And I was working down at the NIH,

which in itself was a real experience in, uh, Steve Rosenberg's lab.

And I was staining up tumor infiltrating lymphocytes and injecting them back

into mice. And they were, there was a, a microenvironment the tumor,

and there was IL two, so they were obviously stimulated. And my,

I was pulling them back out just to see how many got there.

And these damn things were dimm I, and so I'd do another experiment,

stain 'em up, you know, even brighter,

put 'em in and they'd come out and they'd be dimmed. And I probably did this 10,

15 times. And somebody then turned to me and said, well, you know, Paul,

maybe that's a good thing. Maybe they're dividing.

And I went, and actually out of that, um, it wasn't something that I developed,

but the companies in AXIS then went on,

developed something called the Cell Census plus essay now that was looking at,

uh, di dilution and proliferation subsequently CFSE came out. And, you know,

that that sort of took, took a, a part, a good part of the market. But, uh,

I think, you know, getting into the proliferation was,

was something that I look back on as being really proud of. Yeah.

Thank you Jessica.

And yeah, I mean, I might be inclined to say something about my research, like,

because I do fluorescence dynamics and design cytometers to measure lifetimes,

but the first thing that really came to mind was just I feel like I've

contributed because I've been part of the first all female executive committee.

Yeah. There you at.

And I feel like I'm helping to be a role model for the society.

So,

And, and I'm gonna ask, how have you found your first six months,

nine months as president?

Yeah. Mm-Hmm. First, uh, few months now, and we'll be,

continue for a couple more years. Yeah.

And how have you found it? That's an answer for you. It's great.

Okay. So the, so the next question now is, is a very similar question actually.

So the answers may be the same or maybe different. Uh, so from Molina,

what contribution to flow to the flow cytometry society do you think you've made

to the community? Okay.

What is your biggest contribution to the community of flow cytometry? Uh,

I'm gonna go with Paul first.

You know, there's, well,

I think within ISAC I think is what you're referring to. And, uh,

I think within isac, uh, you know, I, I felt like I've really made a,

a con a fair, some significant contributions. One,

and maybe one that really sticks out in my mind, mind,

and this is something that petite mentioned at a, uh,

a council meeting was study youth. So that was something that we really study.

Youth is still going on. That's,

that's something that really impacts on kids and, uh, makes kids aware of, uh,

of, of really what's,

what's going on in flow and really gets them interested in science.

And it was something that we, uh,

developed in Buffalo where we brought kids in from the high school.

We actually put a flow cytometer in the high schools and, uh, well, the schools,

so in eighth grade they were learning,

or fifth grade they were learning about health.

And we would talk about CD fours and demonstrate some of that on the flow.

And then, uh, uh, in eighth grade,

they'd get it again and we'd get into physics. So, you know, there's, there's a,

there's a couple things, but I think, um, you know,

cyto Asia is another one that, uh, I was particularly proud of. But, uh, uh,

cyto youth and the way that's continued is good.

Okay. Uh, petite.

Um, I think it's, uh, it's the, it's an unusual answer,

but it's the spirit that I hope I'm bringing to working with people in

the flow community, that it's, you know,

I'm not trying to keep anything for myself or, or, you know,

I'm just trying to be open with what I know and what I understand and be eager

to help, you know. And, um, that was something I learned from,

from Gary Nolan and from, um, from Mario. And, you know,

um, it's,

it's a great way to generate really positive feelings.

And so when I walk around here and I get to see people that I've worked with,

you know, it's genuine. The happiness of, of interacting with them. And that's,

that's one of the reasons this meeting so much fun to come to. Mm-Hmm.

Thank you. And

Yeah, and just real quick, I mean,

I think I kind of sort of answered that in the last question,

but the cytometry community goes far and wide and deep. And I think,

you know, I can think of a span of things that I do. You know,

I really appreciate working on the associated editorial board for the journal

and then doing things like serving a term in the cell and molecular technology

study section for the National Institutes of Health Center for Scientific

Review. 'cause that, you know, you,

you're looking at all of these really novel types of applications and new

technologies and helping, you know, contribute to those becoming something. So,

Okay, so agile. I've just been WhatsApped. Derek, where are I?

Question to the panel.

What city would you love to have side to in

3, 2, 1 petite?

Um, some beach destination. Like let, let's, let's go crazy.

Let's do it in like Fiji or something. You know, something. Gee, yeah.

Really insane. You know, we'll do it on a beach.

We don't need a convention center. Save some money.

Yeah,

Yeah. Okay.

Oh, I always wanted to do it in Hawaii. And that was I gonna, that was,

that was, that was always high on my list.

Yeah. That is so predictable though.

Well, I'm

Sorry. So, so Jessica, what, what are you gonna say?

Well, I, well, haka Mexico, I know. No, I was, I I,

when you had the beach idea, I thought, I immediately thought, hon Yeah,

It would be fun. It's, it's certainly been discussed. Uh,

So, so, okay, so Hawaii, who'd like to go to Hawaii?

Why aren't we going to Hawaii? Edinburg? Goodness sake. There

Was, why are we going to

Hawaii? There

Was a time when, there was a time, wasn't there, Jessica,

when Honolulu was on the list? It was

Oh, it's, it's definitely been on the list and it's been discussed. Yeah.

We only do the president.

We got the money. Okay. So actually, uh, I, I'm gonna, I,

I've got one question from Alfonso. Where's Alfonso?

There we go. Hello, Alfonso. So the question is,

if you were not doing what you do today, oh,

what kind of job do you think you'd be good at? Oh,

I'm gonna go Jessica first.

Um, a mother. I mean, but that's an easy answer. Be, I mean,

I am a mother now and I have three kids and, you know,

but if I wasn't working just home and hanging with the kids,

Okay. Particularly not allowed to say father.

Sorry.

Yeah.

I don't have to say father.

Yeah, no, no, I'm not, I'm not gonna say father. Um, the, my,

my kids could killed me, right?

Um, and it is being recorded. It, it

Would be, yeah, I know. Really. Oh, big mistake. Um,

it would be a journalist and I'm proud to say that my daughter is gonna go to,

um, to, to school, to college in the fall, to be a journalist. So, you know,

at least there's some, um, you know, genetic influence. I think that's,

that's taking over. So I can watch from, from, uh, from the sidelines

Who I'd like to be a nationalist, taking people out on hikes, uh,

into the wilderness and, uh,

be there identifying plants and flowers and geological formations and stuff like

that. That, that, that would've done me very well.

You know, I think I'd like to be a TV host.

You'd

Be very good at it.

So we are coming to the last few minutes.

So I'd like to actually bring up John Tig, who's joining us later to be our dj.

John, coming up, bring your chair forward, please bring it forward.

Bring it forward, bring it forward. So John, the same question to you.

'cause this is no longer obvious, is it? If you could do any other job,

what would you do?

Um, I'd be Peter O'Toole,

He died. Oh,

That's right. Peter died. I guess, uh,

the obvious answer is I would just be a dj. Um, yeah,

music's passion flow cytometry. What I'm good at.

Okay, so, so I'm now gonna go for some quick questions to finish the night.

This is the, the, so I'm gonna start this time, it's gonna go Paul, Jessica,

petite John,

who is the best place Cytometrist for your generation

Of my generation? Um, well, I, oh gosh. Um,

Of my, of my generation. I think I am, I mean,

I can see what the answers are gonna be now. Jessica,

This is hard. I think petite's my generation petite

Well

Say. You say you.

Yeah, Jessica,

I mean, I, I can totally

Cop out here, you know. I know, but I, I think, I think that this isn't,

this isn't a fair question because we are all doing really,

really cool work in our own fields and we are each our own stars.

There you go. How's that? How's,

That's good.

I'm Joan,

There's no way to follow that up. Um,

'cause any answer now seems very ridiculous. Um, I agree, right? Petite,

very good. You, you, you've made it easy for me. So I agree with petite.

We all are individuals and great in our own way.

Okay, so, so this is being recorded today,

so I'm now gonna go from that side to this side.

What is your favorite streaming device?

My favorite streaming device? Yeah. What do you mean? Like as in an iPhone or

Your Yeah,

We're streaming. Come on. What's streaming? Come

On. It's probably, um,

it's probably the computer because then I can leave it on and like watch, um,

true crime, actually fascinated by it. And I just leave it on in the background.

Like those nights, I don't wanna turn up the music in the char, you know,

I find out why that lady got strangled. So yeah,

Jessica,

My, probably my iPhone. I mean, I like, I'm really connected to it.

I'm surprised I don't have it with me right now, but

Can't believe no one said Netflix. No one said, I didn't know iTunes.

We've got Oak Creek flowing by the, uh, out, out out the backyard. So I,

I'd like Oak Creek, watching Oak Creek go, but, uh, I actually,

to your question, probably I, I spend more time on the tv, uh,

just running streaming on

The tv. Okay. And John, what would be the right answer?

The right answer is I would go what we said before, the mo flow legacy.

That was a setup. Spoiler alert ball.

I have the cheat sheet.

Okay, so next question. I'm gonna start with Jessica this time,

and I'm gonna go, what's your favorite color?

My favorite color, I guess, uh,

I like blue, but are, are we going down a wavelength or whatever?

Okay. Blue blue's good. Blue. Oh, blue. Oh, oh, crap. Wrong. Waving. Blue. Blue,

Yeah. Uh, 4 0 5.

4 0 5

PE

And flu seed. Yeah. Okay. Okay. And on that note,

I think I have covered most of the questions for tonight and we are bang,

gone 40 minutes,

so I'd just like to thank you everyone for actually being really quiet and it's

been really fantastic to have you here. You've been engaged.

Thanks for sending the questions. A big thank you to our guest tonight.